Abstract:
The study was designed for pharmacological investigation of chloroform fraction of
Wedelia trilobata leaves extract (Family: Asteraceae). The powdered leaves of Wedelia
trilobata were extracted with methanol and then partitioned with petroleum ether,
chloroform and ethyl acetate consecutively. The chloroform fraction was investigated for
total flavonoid content, brine shrimp lethality bio-assay and antimicrobial test. The
fraction contained 228.824mg AAE/g of total flavonoid content which shows that the
fraction have prominent antioxidant property. Screening for cytotoxic properties using
brine shrimp lethality bioassay with tamoxifen (LC50 value of 13.38μg/ml) as positive
control showed that the fraction have considerable cytotoxic potency exhibiting LC50
value 27.17μg/ml. In antimicrobial activity investigation, the chloroform fraction showed
low antibacterial and antifungal activity against the tested organisms compared to
ciprofloxacin (30μg/disc) which was used as positive control. The chloroform fraction
showed moderate cytotoxic activity, potent antioxidant activity and slight antimicrobial
activity. Further investigations are needed for the proper identification and isolation of
these bioactive compounds to produce safer drugs for treatment of harmful diseases.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor in Business Administration of East West University, Dhaka, Bangladesh.
