Abstract:
The objective of this research is to study the phytochemical properties of methanolic
extracts of Enhydra fluctuans, a plant belonging to the Asteraceae family. The
phytochemical evaluation was carried out by performing several test TLCs, preparative
TLC, & IR test. Enhydra fluctuans,a tropical herb, commonly known as helencha or
harkuch,belonging to family Asteraceae, is gaining lot of importance for its therapeutic
potentials. This is an edible semi-aquatic herbaceous vegetable plant with serrate leaves,
grows in abundant quantiy in Bangladesh. The leaves are slightly bitter, cure
inflammation, skin diseases, laxative,bronchitis, nervous affection, leucoderma,
biliousness and good in small pox. The plant possesses nutritional value including- _-
carotene, saponins, cholesterol, glucoside, enhydrin and so on. It is reported that plant
possesses Antioxidant, Hepatoprotective, CNS Depressant, Analgesic and Antidiarrheal
activity.Thin-layer chromatography (TLC) is a mature and very established technique,
frequently used in many fields of applications ranging from natural product analysis to
chemical or pharmaceutical applications. Fourier Transform Infrared Spectroscopy
(FTIR) identifies chemical bonds in a molecule by producing an infrared absorption
spectrum. The spectra produce a profile of the sample, a distinctive molecular fingerprint
that can be used to screen and scan samples for many different components. FTIR is an
effective analytical instrument for detecting functional groups and characterizing
covalent bonding information. TLC & IR test performed to investigate bioactive natural
compounds from E. fluctuansand to investigate the crystals obtained from the crude
extract of the plant .Some bands were observed through these tests.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) of East West University, Dhaka, Bangladesh.