Evaluation of Locomotor Activity & Exploratory Behavioral Activity of Thysanolaena Maxima by Open Field Method

Abstract

The present study was carried out to Evaluate of locomotortivity & exploratory behavioral activity of methanol extract of Thysanolaena maxima on the central nervous system of rodent ice. The plant Thysanolaena maxima has been used for the general promotion of health and longevity by Asian tribal. It is used as a traditional medicine for the treatment of various diseases especially as an antitubercular drug in the hilly areas of this subcontinent.The evaluation of locomotor activity & exploratory behavioral activity of methanol extract of Thysanolaena maxima was carried out by using ‗open field‘ method for the dose of 200mg/kg and 400 mg/kg. A group of mice was kept as positive control and was given diazepam as standard drug.Methanol extract of Thysanolaena maxima 200 mg/kg the central locomotion was started from 2.75± 0.25 at 0 minute and ended in 2.25± 0.75 at 120 minutes. Methanol extract of Thysanolaena maxima 400 mg/kg the central locomotion was started from 2.00± 0.00 at 0 minute and ended in 1.00± 1.00 at 120 minutes. We can see the central movement has decreased for both the dose 200mg/kg and 400 mg/kg as the time proceeds.Methanol extract of Thysanolaena maxima 200 mg/kg the peripheral locomotion was started from 11.00±0.00 at 0 minute and ended in at 120 minutes. Methanol extract of Thysanolaena maxima 400 mg/kg the peripheral locomotion was started from 22.75± 11.30 at 0 minute and ended in 21.75±14.46 at 120 minutes. We can see the peripheral movement has decreased for both the dose 200mg/kg and 400 mg/kg as the time proceeds.Methanol extract of Thysanolaena maxima 200 mg/kg the leaning count was started from 24.5±12.14 at 0 minute and ended in 1.50± 0.86 at 120 minutes. Methanol extracts of Thysanolaena maxima 400 mg/kg the leaning count was started from 10.25± 0.25 at 0 minute and ended in 1.25± 0.47 at 120 minutes. We can see the leaning count has decreased for both the dose 200mg/kg and 400 mg/kg as the time proceeds.Methanol extract of Thysanolaena maxima 200 mg/kg the grooming count was started from 17.50±1.19 at 0 minute and ended in 29.00 ± 3.42 at 120 minutes. Methanol extracts of Thysanolaena maxima 400 mg/kg the grooming count was started from 19.00±5.12 at 0 minute and ended in 29.75±0.62 at 120 minutes. We can see the grooming count has increased for both the dose 200mg/kg and 400 mg/kg as the time proceeds. Methanol extract of Thysanolaena maxima 200 mg/kg the defecation count was started from 1.50± 0.28 at 0 minute and ended in 0.75± 0.25 at 120 minutes. Methanol extracts of Thysanolaena maxima 400 mg/kg the defecation count was started from 1.00± 0.81 at 0 minute and ended in 20.25± 0.28 at 120 minutes. We can see the defecation count has decreased for both the dose 200mg/kg and 400 mg/kg as the time proceeds. The result indicated that the extract decreased locomotor activity which indicates it has CNS depressant activity. However, further studies are needed in order to isolate the active principles responsible for the observed activity.