Abstract:
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder, and the most
predominant cause of dementia in the elderly. Traditionally Citrus macroptera (L.)
has widely been used as antioxidant source. Our aim is to investigate the
cholinesterase inhibitory activities, antioxidant properties as well as thrombolytic
properties of the fruits pulp and peel extracts in the treatment of AD and clotting
disorders. The crude methanol extract was prepared by cold extraction method and
was assessed for acetyl cholinesterase and butyryl cholinesterase inhibitory activity by
the Ellman method; their antioxidant properties were assessed by several assays
including reducing power, scavenging of DPPH and hydroxyl radicals together with
quantitative analysis of endogenous substances. Its thrombolytic potential activity is
determined by clot lysis method. Quantitative analysis displayed higher contents of
phenolics and flavonoids in the crude methanolic fraction. The results revealed that
the ethyl acetate extract, possibly due to its phenolic compounds, exerts potential
antioxidant and cholinesterase inhibitory activities which may be useful in the
treatment of AD. But in case of thrombolytic property it seems not so potential at all.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) in East West University, Dhaka, Bangladesh.