Cytotoxic, Antimicrobial and Antioxidant Activity of Crude Fraction of Ficus Resemosa Leaves Extract

Abstract

The purpose of the study was to evaluate the cytotoxic, antimicrobial and antioxidant activity of crude fraction of Ficus recemosa (Family: Moraceae) leaves extract. The powdered leaves of Ficus recemosa were extracted with methanol and then partitioned with pet-ether, Dichloro methane , ethyl acetate and crude fraction was taken for experiment. The crude fraction was used to evaluate cytotoxic, antimicrobial and antioxidant activities. The cytoxic activity was measured by brine shrimp lethality bioassay. Crude fraction showed cytotoxic activity with LC50 value 58.87μg/ml in brine shrimp lethality test The antimicrobial activity was assessed by disc diffusion method.. In antimicrobial activity investigation the crude fraction showed low to moderate antibacterial and antifungal activity against the tested organism compared to the ciprofloxacin (30μg/disc) that was used as positive control. The fraction contained 27.32 mg AAE/g of total phenolic content and 477.65 mg AAE/g total flavonoid content. The results of study clearly indicate the presence of cytotoxic, antimicrobial and antioxidant properties of crude extract. The obtained results provide a support for the use of this plant in traditional medicine and its further investigation.