Cytotoxix and Antioxidant activity in aqueous fraction of Opuntia elatior extract

Abstract

The purpose of the study was to evaluate the antioxidant and cytotoxic activity of crude extract of Opuntia elatior mill (Family: Cactaceae).

The powdered of Opuntia elatior mill were extracted with methanol and then partitioned with pet-ether, DCM, ethyl acetate and crude fraction was taken for experiment.

The crude extract was used to evaluate cytotoxic and antioxidant activities. The cytotoxic activity was measured by brine shrimp lethality bioassay.

.Screening for cytotoxic properties using brine shrimp lethality bioassay with tamoxifen (LC50 value of 25μg/ml) as positive control showed that the fraction have considerable cytotoxic potency exhibiting LC50 value 6.25μg/ml.

The crude extract contained 23.895 mg AAE/g of total phenolic content, 111.1 mg AAE/g of total reducing power content and 58 mg AAE/g total flavonoid content.

The results of study clearly indicate the presence of cytotoxic and antioxidant properties of crude extract. The obtained results provide a support for the use of this plant in traditional medicine and its further investigation.