In vitro Minimum Inhibitory Concentration(MIC) of Antibiotics Standard Powder Against Different Clinically Isolated pathogenic bacteria & Resistance pattern

Abstract

The conventional broth macrodilution method to test minimum inhibitory concentration (MIC) of antibiotics standard powder against Different Clinically Isolated Pathogenic Bacteria.This study was employed to evaluate the microbial resistance pattern of antibiotics standard powder were Cepharadine, Cefuroxime, Ceftriaxone, Cefixim, Azithromycin, Ciprofloxacin, Levofloxacin & Vancomycin against 6 types clinically isolates 23 strain of pathogenic bacteria (Staphylococcus aureus, Salmonella typhi, Klebsiella spp., Pseudomonas spp., Escherichia coli & Acinetobactor spp.). Where antibiotic standard powder was used in various concentrations (in serial dilution from 0.015625-128μg/ml) and 10µl suspension of test organisms (containing

1.5×106 cells per ml) were taken in the test tubes and mixed well. Three control test tubes CS, CM and CI were used to perform control test .Then the test tubes were incubated at 37.5ºc for 18-20 hrs. The result was measured by observing minimum inhibitory concentration (MIC) value. Here, E.coli has total 9 strains and gives resistant activity against antibiotics standard powder but majority of the strains sample gives highest sensitivity MIC values that are Levofloxacin (0.015625μg/ml) for sample 9, Ciprofloxacin (0.03125μg/ml) for sample 1, Cepharadin (0.5μg/ml) for sample 2, Ceftriaxone (0.0625μg/ml) for sample 5 & Azithromycin (0.0625μg/ml) for sample 7. E.coli sample 4 is resistant against all antibiotics. Here, Klebsiella spp. total 4 strains sample some gives highest sensitivity MIC values that are Levofloxacin (0.0625μg/ml) for sample 10, Ciprofloxacin (0.25μg/ml) for sample 10, Cepharadin (2μg/ml) for sample 10 & Azithromycin (0.0625μg/ml) for sample 13. Staphylococcus aureus total 2 strains some gives highest sensitivity MIC values that are Levofloxacin (0.0625μg/ml), Cepharadine (0.125μg/ml), Cefuroxime (0.5μg/ml), Ceftriaxone (0.5μg/ml) & Azithromycin (0.0625 μg/ml) for sample 14. Acinetobector spp. has 1 strain gives a highest sensitivity MIC values that are Levofloxacin (0.5μg/ml) & Ceftriaxone (4μg/ml) for sample 16. Pseudomonas spp. total 3 strains some gives highest sensitivity MIC values that are Levofloxacin (1μg/ml) for sample 19, Ciprofloxacin (0.03125μg/ml) for sample 17 & Azithromycin (1μg/ml) for sample 17. Pseudomonas spp. sample 18 is resistant against all antibiotics. Salmonella typhy total 4 strains some gives highest sensitivity MIC values that are Levofloxacin (0.0625μg/ml) for sample 21, Ciprofloxacin

(0.125μg/ml) for sample 20 & 22, Cepharadin (0.25μg/ml) for sample 20 & 22, Ceftriaxone (1μg/ml) for sample 21 & Azithromycin (0.25μg/ml) for sample 23.