"In Vitro Minimum Inhibitory Concentration (MIC) of Antibiotics Against Clinically Isolated Bacteria"

Abstract

MIC is the lowest concentration of an antibiotic required to inhibit the growth of an organism.MIC was done against eight conventional antibiotics e.g. Cephradine, Cefuroxime, Ceftriaxone,Cefixime, Vancomycin , Azithromycin , Ciprofloxacin, Levofloxacin. Enterococci sp, E.coli , Pseudomonus, Klebsiella, were collected from hospital . Antibiotics were subjected to sensitivity test against this clinical isolates. Seventeen autoclaved test tube were taken, of which nine were marked 1 to 14 and the rest three were assigned as CM (medium), CS (medium+ sample) and CI (medium+ inoculums). Then 10μl of the diluted inoculums of organism (1.5×106cells/ml) was added to each of the fourteen test tubes and mixed well. The terbidity of Inoculums must be same as McFarland Standard. The results compelled to the standard value of CLSI, EUCAST . Most of the time cephalosporine group antibiotics showed resistance against clinically isolated bacteria , but Ceftriaxone from this group with MIC range 0.5mg/L ,compelled to the standard value of CLSI, against E.coli sample 3 , so it is considered effective against this strain of E.coli . The highest MIC value for levofloxacin was 0.0625 mg/L against E.coli sample 3. levofloxacin also effective against E .coli sample 4, sample5 & sample 1 with MIC value 0.25mg/L ,0.25 mg/L & 2 respectively . The highest MIC value for ciprofloxacin against E.coli sample 4 & sample 5 was 0.5 mg/L The highest value of pseudomonas against Levofloxacin & Ciprofloxacin was 0.5 mg/L & 0.0625 mg/L respectively for sample 1. The highest value for Klebsiella sample 1 & sample 4 against Levofloxacin was 0.5 mg/L & 0.25 respectively. The highest MIC value for Klebsiella sample 1 & sample 4 against Ciprofloxacin was o.25 mg/L & 1 mg/L respectively .Ceftriaxone also gives activity with MIC range 0.5 mg/L against Klebsiella sample 1 , otherwise Macrolide group antibiotic Azithromycin & cell wall synthesis inhibitors Vancomycin is totally resistance against all strains of klebsiella & E.coli . Enterococci & Acinobactor sp. was totally resistance against our used antibiotics.