In Vitro Sensitivity of Carca Pappya Seed Extract Against Clinical Isolates of Entamoeba Histolytica

Abstract

Background: Entamoeba histolytica is the etiological agent of amoebic dysentery and amoebic liver abscess. Amoebiasis is one of the most common health problems in the developing countries. Almost 10% people of the world suffer from amoebiasi s. Poverty, ignoranc, overcrowding, poor sanitation and malnutrition favor transmission and increased disease burden. Objectives: To find out the in vitro sensitivity of Carica papaya seed extract against clinical isolates of Entamoeba histolytica. Materials and methods: Entamoeba histolytica isolates collected from the stool and cultured in different culture medium. Carica papaya seed extract prepared in different concentrations of 0.12 mg/ml, 0.24 mg/ml, 0.49 mg/mI, 0.98 mg/ml, 1.97 mg/ml, 3.95 mg/ml and 7.98 mg/ml using the Di-methyl sulphoxide (DMSO) solvent and incubated at 24 hours and 48 hours respectively. After incubation the isolates of viable Entamoeba histolytica were calculated.Result: Viable count of the Entamoeba histolytica in each concentration of Carica papaya seed extract was compared to the initial concentration and with the control. After 24 hours of incubation concentration of 7.89 mg/mI shows no presence of Entamoeba histolytica. After 48 hours of incubation concentrations of 0.98 mg/mI, 1.97 mg/mI, 3.95 mg/mI, 7.89 mg/mI shows no presence of the clinical isolates of Entamoeba histolytica. Conclusion: The results show that the in vitro sensitivity of Carica papaya seed extract against the Clinical isolates of Entamoeba histolytica.