In-vitro Assessment of Cholinesterase Inhibitory and Antioxidant Activity of Solanum indicum plant extracts

Abstract

Reduced level of acetylcholine (ACh) is an important hallmark of Alzheimer’s disease (AD), a common type of progressive neurodegenerative disorder. Effective treatment strategies rely mostly on either enhancing the cholinergic function of the brain by improving the level of ACh from being a breakdown by cholinesterase enzymes. Traditionally Solanum indicum (L.) has widely known for its medicinal values. Our aim is to investigate the cholinesterase inhibitory activities, Antioxidant activities of the whole plant extracts in the treatment of AD and clotting disorder. The crude methanol extract was prepared by cold extraction method and other fractions are collected by liquid liquid partitioning. To determine acetylcholinesterase (AChE) inhibitory activity Ellman’s method was applied. The plant is found moderate active in inhibiting Acetylcholinsterase enzyme, as it inhibits 43.95% at 200μg/ml. Its total phenolic content, total antioxidant capability and free radical scavenging capability is also very high for this plant. But this plant lacks in containing flavonoids. of further investigations to identify the promising molecule(s) potentially useful in the treatment of AD.