Phytochemical and Pharmacological Investigation on Phyllanthus Acidus Leaf

Abstract

The purpose of the study was to evaluate the cytotoxic, antimicrobial and antioxidant activity of n-hexane fraction of Phyllanthus acidus leaves extract. The powder of Phyllanthus acidus were extracted with methanol and then partitioned with petroleum ether, DMSO, ethyl acetate and crude fraction was taken for experiment. The n-hexane fraction was used to evaluate cytotoxic, antimicrobial and antioxidant activities. The cytotoxic activity was measured by brine shrimp lethality bioassay. LC50 value of n-hexane fraction of Phyllanthus acidus leaves is 158951614.2 μg/ml in brine shrimp lethality test. The fraction contained 4.5 mg AAE/gm of total phenolic content, 4.868 mg of Quercetin/gm of total flavonoid content. The results of study clearly indicate the presence of very poor cytotoxic and poor antioxidant properties of n-hexane extract. The study also showed low to moderate antimicrobial activity. The obtained results provide a support for the use of this plant in traditional medicine and its further investigation.