Abstract:
A simple, precise, accurate and rapid UV Spectroscopic method has been developed for the assay of Ciprofloxacin hydrochloride (HCl) in its pharmaceutical tablet dosage form. The UV Spectroscopic method was validated according to the International Conference on Harmonisation (ICH) guideline, where range, linearity, accuracy, recovery, precision and sensitivity of the method were examined. Ciprofloxacin HCl showed maximum wavelength of absorbance at 272 nm in water and obeyed linearity or Beer-Lambert’s law within the concentration range of 2-12 μg/mL. The regression of coefficient (R2) was found to be greater than 0.99. The limit of detection (LOD) and limit of quantitation (LOQ) values were found to be 0.1 μg/mL and 2 μg/mL, respectively. The developed method was applied successfully for the analysis of ciprofloxacin HCl with good accuracy and precision.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) in East West University, Dhaka, Bangladesh.
