Abstract:
The study was designed for pharmacological investigation of ethyl acetate (EA) fraction of
methanol extract of the leaves of Ficus hispida (Family: Moraceae). The powdered leaves of
Ficus hispida were extracted with methanol and then partitioned with n-hexane, dichloromethane
and ethyl acetate consecutively. The EA fraction remaining was investigated for total flavonoid
content, total phenol content, brine shrimp lethality test. The fraction contained 139.167mg
AAE/gm of dried extract in total phenolic content assay and 9.73mg quercetin/gm of dried
extract in total flavaniod content assay. Screening for cytotoxic properties using brine shrimp
lethality bioassay with tamoxifen (LC50 value of 35.23 μg/ml) as positive control showed that the
fraction have considerable cytotoxic potency exhibiting LC50 value of 1.79 μg/ml. The EA
fraction showed strong cytotoxic activity, low antioxidant activity and low to moderate
antimicrobial activity. Further investigations are needed for the proper identification and
isolation of these bioactive compounds to produce safer drugs for treatment of harmful diseases.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) in East West University, Dhaka, Bangladesh.
