Study of Pharmacological Activities of Methanolic Extract of Aglaonema hookerianum Leaf

Abstract

Purpose: The research work was carried out to determine the pharmacological activities of methanolic extract of Aglaonema hookerianum Method: Methanolic extract was administered orally to the animal model (Swiss albino) and the effects were determined by comparing with respect to control group which were treated with 5% CMC. For every experiment positive control was used. For every experiment positive control was used. Different experiments were used to determine the pharmacological profile which was Collected from internationally published publications and journals. Result: The Laxative Effect was evaluated by Charcoal meal GI transit test where methanolic extract at dose of 800 mg/kg body weight increase the charcoal transit which compare with positive control. The CNS activity was evaluated by open field method and hole board test. In the open field method and hole board experiment the crude extract of Aglaonema hookerianum. (200mg/kg, 400mg/kg & 800mg/kg) dose dependently reduces the number of peripheral locomotion, central locomotion and leaning in the open field test but increases the number of grooming. The reduction is significant (***p<0.001) when it is compared to the standard drug. In the methanolic extract of Aglaonema hookerianum(Leaf) it showed highest 41.75% thrombolytic activity and moderate activity showed by the above experiment compared with the streptokinase and Aglaonema hookerianum( Bark). In total phenolic content assay of methanolic extact of Aglaonema hookerianum have the phenolic content of (122.6 mgSAE/g). In DPPH radical activity of methanolic extract of Aglaonema hookerianum is good (IC50 11.0(μg/ml). In reducing power assay higher absorbance of the reaction mixture indicates higher reductive potential. In this assay, Aglaonema hookerianum shows as the concentration increases the absorbance is also increase . The aim of the study was also to investigate the possible toxicity of the plant Aglaonema hookerium and especially to establish the safety of the methanolic extract of this plant by focusing on its acute and chronic toxicity in mice. For finding chronic toxicity several tests are done such as CBC (Cell Blood count) test, Hepatic enzyme test and histopathological Studies. All data were analyzed by using SPSS analytical method. Conclusion: After summarize all the results it can say that bark of Aglaonema hookerium may have several pharmacological activities but to prove the hypothesis it need further higher studies.