Abstract:
The study was designed for pharmacological investigation of dichloromethane (DCM) fraction
of methanol extract of the leaves of Ficus hispida. The powdered leaves of Ficus hispida were
extracted with methanol and then partitioned with n-hexane, dichloromethane and ethyl acetate
consecutively. The DCM fraction remaining was investigated for total flavonoid content, total
phenol content, brine shrimp lethality test. The fraction contained 139.167 mg AAE/gm of dried
extract in total phenolic content assay and 35.3396mg quercetin/gm of dried extract in total
flavaniod content assay. Screening for cytotoxic properties using brine shrimp lethality bioassay
with tamoxifen (LC50 value of 0.78726 μg/ml) as positive control showed that the fraction have
considerable cytotoxic potency exhibiting LC50 value 23988329194.9045 μg/ml. The DCM
fraction showed weak cytotoxic activity, low antioxidant activity. Further investigations are
needed for the proper identification and isolation of these bioactive compounds to produce safer
drugs for treatment of harmful diseases.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) in East West University, Dhaka, Bangladesh.
