In Vitro Antimicrobial Susceptibility Test of Ciprofloxacin hydrochloride- 500mg (Ciprocin® and Ciprofloxacin®)

Abstract

Ciprofloxacin is a fluroquinolone antibiotic act against a broad spectrum of bacteria including gram positive and negative. It is effective against urinary tract infection, acute prostatitis, sinusitis, lower respiratory tract infection etc. To get therapeutic effect in maximum level the quality of the drug need to control. In a pharmaceutical sense, quality means checking and directing the degree or grade of excellence of processes and products. Antimicrobial susceptibility test is one of the quality control tests to estimate the possible effectiveness of antibiotics. It is performed by using disk diffusion test. The objective of the study is to measure the sensitivity of the antibiotic by comparing with the standard. It is significant because of the rapid occurrence of antibiotic resistance due to use of substandard or poor quality drugs or due to the irrational use of drug. The test was performed using Ciprocin® and Ciprofloxacin® 500mg Tablet in concentration of 5μg/disc. Different species of bacteria such as three different strains of E. coli and two strains of Pseudomonas aeruginosa, Salmonella typhi were subcultured in agar plate which was isolated from the urine, stool and blood sample of the pediatric patients. Different concentrations of sample were applied by disc diffusion method and incubated overnight. Inhibitory activity of the antibiotic was measured as zone of inhibition in mm. The zone of inhibition of the sample was compared with the zone of inhibition of the standard. Results show that both Ciprocin ® and Ciprofloxacin® 500mg possibly contain the sufficient amount of active ingredient which is required to achieve desired therapeutic activity. It was the possible indication of drug effectiveness which was almost similar with the standard. Further studies need to confirmation of results.