Biological Investigation of the Ethanolic Extract of Polygonum Hydropiper (L), Coccinia Grandis (L) and Litsea Glutinosa (Lour.)

Abstract

Polygonum hydropiper, Coccinia grandis and Litsea glutinosa are common plants in Bangladesh. It has been reported to Coccinia grandis have medicinal activities like hypoglycemic, antibacterial, antifungal and Litsea glutinosa used for used as demulcent for ulcer; also for dysentery. The leaf of Polygonum hydropiper plant has shown cytotoxic and thrombolytic activity. In this study, extracted mixture from Polygonum hydropiper, Litsea glutinosa and Coccinia grandis, were studied for antibacterial, thrombolytic, cytotoxic and scavenging activity by using in vitro techniques. Analgesic activity was tested using in vivo techniques. Ethanol extract of the plants were tested for Antibacterial activity using disc diffusion method. Ethanolic extract of the plants were tested for brine shrimp lethality bioassay using brine shrimp nauplii. The LC50 of ethanol extract of Polygonum hydropiper extract was found to be 6.02μg/ml indicates that extracts have good pharmacological action. The ethanol extract of the extracts were also tested for in vitro thrombolytic activity. The extract showed reasonable thrombolytic activity. Litsea glutinosa showed very good analgesic activity having percent inhibition of writhing about 76.65. Coccinea grandis has extremely good scavenging activity having the IC50 of 1.67μg/ml. Further investigation on the plant is required to confirm their pharmacological activity and thereby utilizing them as useful medicinal plant.