In Vitro Anti-Microbial Evaluation of Different Crude Extracts from Dillenia Pentagyna Leaf and Stem

Abstract

Background: Dillenia pentagyna is widely distributed in tropical regions of Asia. The traditional use of this plant suggests its possible antimicrobial properties, but its efficacy has not been examined yet. Objective: The purpose of this research was to evaluate the antimicrobial efficacy of crude extracts of Dillenia pentagyna leaf and stem against pathogenic microorganism using the disk diffusion method. . In this study, the crude ethyl acetate, ethanol & water extract of both leaf and stem was assessed. Materials and methods: Two different concentrations of 800μg/disc and 400μg/disc for crude leaf and stem extracts of Dillenia pentagyna were tested for antimicrobial efficacy against five Gram-positive (Bacillus sereus, Bacillus subtilis, Bacillius megaterium, Staphylococcus aureus & Sarcina lutea), eight Gram negative bacteria( Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Salmonella paratyphi, Shigella dysenteriae, Shigella boydii, Vibrio mimicus & Vibrio parahemolyticus) and two fungi (Candida albicans & Saccharomyces cerevisiae). The antimicrobial activity of the extracts was observed against the standard antimicrobial agent Cephradine of 30 μg/disc. The sensitivity of plant was tested using the disk diffusion method by observing and measuring the zone of inhibition for each sample against each microorganism. Results: Maximum activity was revealed by ethyl acetate extract of the stem followed by the ethanol extract. The ethyl acetate and ethanol extract of leaf showed more or less sensitivity almost against all pathogens. The water extracts revealed very less activity against the microorganisms. Conclusion: This plant may be effective for treatment of different pathogenic diseases.