Anti-oxidant and Analgesic Activity of Phragmipedium Longifolium

Abstract

In vivo Analgesic and in vitro Antioxidant effects of the ethanolic extract of Phragmipedium Longifolium were tested in this project. In Anti-oxident activity test of ethanolic extract of Phragmipedium Longifolium by quantitative DPPH method shows very good anti-oxidant activity. Result showed significant analgesic activities compared to control and the IC50 is 63.8μg/ml compare to control ascorbic acid (IC50 10.81μg/ml). I hope further research on this plant can give a very good anti-oxidant source. In Analgesic activity test of ethanolic extract of Phragmipedium Longifolium by Acetic acid induced writhing investigated method in mice, positive control group (Diclofenc-Na) showed writhing of 7.2±1.923538, (P<0.001) and the experimental group 200 mg/kg body weight dose group showed writhing with average of 4±1.581139. Further study is necessary to find out the active ingredient responsible for analgesic activities.