Abstract:
Azithromycin is a macrolide antibiotic used in Bangladesh, India & other parts of the world as the treatment of bacterial infections, most often those causing middle ear infections, strep throat, pneumonia, typhoid, bronchitis, sinusitis etc. It is derived from erythromycin. It has a similar antimicrobial spectrum like erythromycin. It is more effective against certain gram-negative bacteria than erythromycin. The purpose of this research was to identify antibacterial activity of different brands of azithromycin.
To give a small dose & to inhibit the bacterial growth to know the MIC is very important to
know. Azithromycin of the company Beximco, Orion, ACI, Navana & Opsonin were used in
this research. We identified the antibacterial activity of these brands to know the MIC using disk diffusion method by measuring the zone of inhibition in cm range. According to this method the bacterial isolation was isolated at a standard concentration with 0.9% NaCl. This isolation was given on the agar plate. Nutrient agar is used as agar medium. Here ethanol was used as antiseptic to clean the spreader at the time of spreading the bacterial isolation. Commercially prepared disks were dispensed onto the agar surface on agar plate. Before dispensing each of the disks was impregnated with that standard concentration of a particular antibiotic. An overnight incubation was done to observe the bacterial growth around each disk. Using scale the zone of inhibition was measured in cm range to know the better antibacteial activity of brand of azithromycin of those five companies. The higher data represented the MIC of a specific brand comparing with the data of azithromycin as standard & proves the better antibacterial activity of that brand.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) of East West University, Dhaka, Bangladesh.
