Extraction, Identification and Estimation of Caffeine & Catechin from Corchorus capsularis leaves extract

Abstract

Medicinal plants are the most important source of life saving drugs for the majority of world‟s population. People of all countries in the world have used hundreds to thousands of indigenous plants. Jute (Corchorus capsularis) is the most widely cultivated species for its nutritious leaves, bark, root, & Stems etc. The objective of this study was to isolate, identify and quantify caffeine & catechin from (Corchorus capsularis) leaves extract. Caffeine was isolated from Corchorus capsularis leaves extract and was identified and quantified by phytochemical analysis of TLC (Thin layer chromatography technique), by determined caffeine‟s spot, UV detection in spectrophotometer with ƛmax- 260 n.m, measured caffeine‟s amount in 1mg jute leaf (0.234 mg), total phenolic content method detected phenol‟s amount in isolated caffeine (50.26 mg/g). The isolated caffeine was tested for antimicrobial and antioxidant activities, & results showed no antimicrobial activity and good antioxidant activity with IC 50 values of (285 μg/ml), and Brine shrimp assays exert moderate cytotoxic effect. Isolated caffeine‟s every experiment was compared with pure caffeine. Isolated caffein showed more cytotoxicity & better antioxidant activity then pure caffeine but similarity showed in antibacterial test, both pure & Isolated caffeine have no antibacterial effect. R.F (Retardation factor) of pure caffeine was 0.66 c.m & isolated caffeine was 0.98 c.m. Catechine was isolated from Corchorus capsularis leaves extract and was identified and quantified by phytochemical analysis of (TLC) Thin layer chromatography technique. Catechin were tested for antimicrobial and antioxidant activities, brine shrimp lethality assays etc. Result of isolated catechin shows individual peak in U.V spectrometer with ƛmax- 264 & 320 n.m, well antioxidant effect with IC 50 values of (432 μg/ml), no antibacterial but moderate cytotoxicity observed by brine shrimps lethality tests (Compared with acetone because catechin was found jute leaves lower layer acetone fraction). Total phenol content method, detected phenol‟s amount in isolated catechin (44.25 mg/g). R.F (Retardation factor) of isolated catechin was 0.94.c.m