Investigation of Phytochemical and Cytotoxic Activity of Methanol Extract of Spondias pinnata Bark

Abstract

Spondias pinnatais a medicinal herb belonging to the family Family- Anacardiaceae also known as wild mango, hog-plum, which has been used to dysentery, diarrhea, stomach ache, rheumatism properties according to indigenous systems of medicine in this sub continent. But its antioxidant, antimicrobial, cytotoxic potential and phytochemical screening is still unknown. Therefore, the present study was designed to investigate antioxidant, antimicrobial and cytotoxic properties and to investigate the phytochemical compounds of spondias pinnata. For phytochemical screening, we found the presence of alkaloids, flavonols, phenols and saponins. In order to investigate the antioxidant property, we are experiencing DPPH test, Total Reducing power test and Total phenol content test. In DPPH scavenging IC50 value of extract was 397 whereas the IC50 value of standard was 409. The Reducing power test showed lower reducing potential of extract compared to standards which indicates the slightly presence of phenol and polyphenolic compounds. For example, in the concentration of 1000 (μg/ml) the reducing potential of extract was 8.58% and of the standard was 24.93 %. In Brine shrimp lethality test the LC50 of the extract was 0.362 and the LC50 of the standard was 1.412 which showed lower cytotoxic activity compare to standards.