Abstract:
Amebiasis is a very common disease allover the world. Almost 10% people of the
world suffer from amoebiasis. Poverty, ignorance, overcrowding, poor sanitation and
malnutrition favor transmission and increased disease burden. Entamoeba histolytica is
the etiological agent of amoebic dysentery and amoebic liver abscess. The objective of
this study was to find out the in vitro sensitivity of Nitazoxanide against clinical isolates
of Entamoeba histolytica. Entamoeba histoiytica isolates were collected from the stool
and then cultured in different culture medium. Zoxanide was prepared in different
concentrations of 4.6, 2.3 ,1.15 , 0.575 , 0.287 ,0.143 and 0.07187 J.1M using the Dimethyl
sulphoxide (DMSO) solvent. They were incubated for 24 hours at 37°C. After
incubation the isolates of viable Entamoeba histoiytica were counted. The viable counts
of the Entamoeba histolytica in each concentration of Zoxanide were compared to the
initial concentration as well as the control. As the concentrations of Nitazoxanide
decreases (4.6, 2.3, 1.15, 0.575, 0.287, 0.143 and 0.07187 flM), the inhibitory activity of
Zoxanide was also found to be decreased. Further studies are needed to check the efficacy
of other brands ofNitazoxanide.
Description:
This thesis submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy (B.Pharm) in East West University, Dhaka, Bangladesh.
